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rabbit mab anti erg cell signaling technology cat  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit mab anti erg cell signaling technology cat
    Rabbit Mab Anti Erg Cell Signaling Technology Cat, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/erg/LEF1+Rabbit+mAb/pm41916278-260-15-18
    Average 96 stars, based on 310 article reviews
    rabbit mab anti erg cell signaling technology cat - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: UBX‐390: A Novel Androgen Receptor Degrader for Therapeutic Intervention in Prostate Cancer
    Article Snippet: The chemicals used in this study were DHT (D‐073; Sigma‐Aldrich), IL‐2 (PHC0021, Gibco, Waltham, MA, USA), bortezomib (S1013; Selleckchem, Houston, TX, USA), MLN4924 (5.05477.0001; Millipore, Burlington, MA, USA), bafilomycin (19‐148; Millipore), pomalidomide (S1567; Selleckchem), and cycloheximide (C4859; Sigma–Aldrich). .. Primary antibodies against the following proteins were used for immunoblotting: GR (A303‐490A) and ELL2 (A302505A) from Bethyl Laboratories (Montgomery, TX, USA); PR (sc‐810), VHL (sc‐17780), PCLAF (sc‐390515), and hemagglutinin‐tag (sc‐7392) from Santa Cruz Biotechnology (Dallas, TX, USA); ER (8644), AR (5153S), glyceraldehyde‐3‐phosphate dehydrogenase (2118S), c‐myc (5605S), NKX3.1 (83700S), ERG (97249S), lamin B (17416S), α‐tubulin (2125s), and vinculin (4650S) from Cell Signaling Technology (Danvers, MA, USA); CRBN (NBP1‐91810) from Novus Biologicals (Centennial, CO, USA); CRISP‐3 (AF2397) from R&D Systems (McKinley Place NE, MN, USA); KLK2 (ab152136), SCD1 (ab39969), and BNIP3 (ab109362) from Abcam (Cambridge, UK). .. Total lysates were separated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes using a Trans‐Blot Turbo Transfer kit (Bio‐Rad Laboratories, Hercules, CA, USA).

    Chromatin Immunoprecipitation:

    Article Title: Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For WB: p300 (1:500, Invitrogen: MA1-16608, RW168, Vendor-validated); CBP (1:500, Invitrogen: PA5-27369, , Vendor-validated); AR (1:1000, Abcam: ab133273, ER179(2), Vendor-validated); H2BK5ac (1:1000, Cell Signaling Technology: 12799S, Vendor-validated); H2BK20ac (1:1000, Cell Signaling Technology: 34156S, vendor-validated); H2BK12ac (1:1000, Abcam: ab40883, EP858Y, Vendorvalidated); H2BK16ac (1:1000, Abcam: ab177427, EPR17598, Vendor-validated); H3K27ac (1:1000, Cell Signaling Technology: 8173S, D5E4, Vendor-validated); H3K18ac (1:1000, Active Motif: 39755, Vendor-validated); H3K4me1 (1:1000, Abcam: ab8895, Vendorvalidated); H3K4me3 (1:1000, Active Motif: 39060, Vendor-validated); H3K27me3(1:1000, Millipore: 07-449, Vendor-validated); H2B (1:1000, Active Motif: 39210, Vendor-validated); H3 (1:1000, Cell Signaling Technology: 3638S, 96C10, Vendor-validated); H2BK120ac (1:1000, Active Motif: 39119, Vendor-validated); Myc (1:1000, Cell Signaling Technology: 9402S, Vendor-validated); KLK3/PSA (1:1000, Dako: A0562, Vendor-validated); CITED2 (1:1000, Abcam: ab108345, EPR3416(2), Vendor-validated); NKX3-1 (1:1000, Cell Signaling Technology:83700S, D2Y1A, Vendor-validated); CCND1 (1:1000, Abcam: ab16663, SP4, Vendor-validated); FOXA1 (1:1000, Thermo Fisher Scientific: PA5-27157, Vendor-validated); Vinculin (1:1000, Cell Signaling Technology: 18799S, E1E9V, Vendorvalidated) ; GAPDH (1:1000, Santa Cruz Biotechnology: sc-47724, 0411, Vendor-validated); BRD2 (1:1000, Bethyl Laboratories: A700-008,BL-167-2A2, Vendor-validated); BRD3 (1:1000, Bethyl Laboratories: A302-368A, Vendor-validated); BRD4 (1:1000, Bethyl Laboratories: A700-004CF, BL-149-2H5, Vendor-validated); GSPT1 (1:1000, Proteintech: 28130-1-ap, Vendor-validated); Aiolos (1:1000, Cell Signaling Technology: 15103S, D1C1E, Vendor-validated); Ikaros (1:1000, Cell Signaling Technology: 14859S, D6N9Y, Vendor-validated), HDAC3 (1:1000, Abcam, ab137704, Vendor-validated), Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:10000, Biorad, #1706515), ECL Peroxidase (HRP) Anti-Mouse IgG (1:10000, Cytiva,NA931-100UL). .. For ChIP-seq: H3K27ac (1ug/million cells, Diagenode: C15410196, Vendor-validated); p300 (2ug/million cells, Abcam: ab14984, G230 / NM-11, Vendor-validated); H2BK20ac (1ug/million cells, Cell Signaling Technology: 34156S, vendor-validated); H2BK5ac (1ug/ million cells, Cell Signaling Technology: 12799S, Vendor-validated); H2BK16ac (1ug/million cells, Abcam, ab177427, EPR17598, Vendor-validated); AR (2ug/million cells, Millipore: 06-680, Vendor-validated); FOXA1 (2ug/million cells, Thermo Fisher Scientific: PA5-27157, Vendor-validated); BRD4 (2ug/million cells, Diagenode: C15410337, Vendor-validated); H3K18ac (1ug/million cells, Active Motif: 39755, Vendor-validated); MED1 (2ug/million cells, Active motif: 61065, Vendor-validated); ERG (2ug/million cells, Cell Signaling Technology: 97249S, A7L1G, Vendor-validated); RNA Pol II (1ug/million cells, Active motif: 39097, 4H8, Vendor-validated); Drosophila H2AV antibody for ChIP-seq Spike-in reference (1ug/25ug chromatin, Active Motif: 61686, Vendor-validated) For IHC or IF: p300 (1:250, Invitrogen: 33-7600, NM11, Vendor-validated); CBP (1:100, Invitrogen: PA5-27369, Vendor-validated); AR (1:4000, Abcam: ab133273, EPR1535(2)); Ki67 (predilute, Ventana Medical Systems: 790-4286, 30-9, Vendor-validated); H2BK20ac (1:50, Abcam: ab177430, epr859, Vendor-validated); H2BK5ac (1:250, Abcam: ab40886, Vendor-validated); CCND1 (predilute, Cell Marque: 241R-18, SP4, Vendor-validated); KRT8 (1:50, Abcam: ab53280, EP1628Y); H3K27ac (1:200, Cell Signaling Technology: 8173S, D5E4, Vendor-validated), Anti-rabbit HRP (predilute, .OmniMap, 760-4311), Anti-mouse HRP (predilute, OmniMap, 760-4310); nature portfolio | reporting sum m ary April 2023 4 nature portfolio | reporting sum m ary April 2023 Validation All antibodies used in this study are from reputed commercial vendors and have been validated by the vendors (see website). ..

    Article Title: p300/CBP degradation is required to disable the active AR enhanceosome in prostate cancer
    Article Snippet: For immunoblotting, the following antibodies were used: p300 (Invitrogen: MA1-16608); CBP (Invitrogen: PA5-27369); AR (Abcam: ab133273); H2BK5ac (Cell Signaling Technology: 12799S); H2BK20ac (Cell Signaling Technology: 34156S); H2BK12ac (Abcam: ab40883); H2BK16ac (Abcam: ab177427); H3K27ac (Cell Signaling Technology: 8173S); H3K18ac (Active Motif: 39755); H3K4me1 (Abcam: ab8895); H3K4me3 (Active Motif: 39060); H3K27me3(Millipore: 07-449); H2B (Active Motif: 39210); H3 (Cell Signaling Technology: 3638S); H2BK120ac (Active Motif: 39119); Myc (Cell Signaling Technology: 9402S); KLK3/PSA (Dako: A0562); CITED2 (Abcam: ab108345); NKX3-1 (Cell Signaling Technology:83700S); CCND1 (Abcam: ab16663); FOXA1 (Thermo Fisher Scientific: PA5-27157); Vinculin (Cell Signaling Technology: 18799S); GAPDH (Santa Cruz Biotechnology: sc-47724); BRD2 (Bethyl Laboratories: A700-008); BRD3 (Bethyl Laboratories: A302-368A); BRD4 (Bethyl Laboratories: A700-004CF); GSPT1 (Proteintech: 28130-1-ap); Aiolos (Cell Signaling Technology: 15103S); Ikaros (Cell Signaling Technology: 14859S). .. For ChIP-seq, the following antibodies were used: H3K27ac (Diagenode: C15410196); p300 (Abcam: ab14984); H2BK20ac (Cell Signaling Technology: 34156S); H2BK5ac (Cell Signaling Technology: 12799S); AR (Millipore: 06-680); FOXA1 (Thermo Fisher Scientific: PA5-27157); BRD4 (Diagenode: C15410337); H3K18ac (Active Motif: 39755); MED1 (Active motif: 61065); ERG (Cell Signaling Technology: 97249S); RNA Pol II (Active motif: 39097); H3K18ac (Active Motif: 39755). .. For immunohistology and immunofluorescent staining, the following antibodies were used: p300 (Invitrogen: 33-7600); CBP (Invitrogen: PA5-27369); AR (Abcam: ab133273); H3K27ac (Abcam: ab4729); Ki67 (Ventana Medical Systems: 790-4286); H2BK20ac (Abcam: ab177430); H2BK5ac (Abcam: ab40886); CCND1 (Cell Marque: 241R-18).

    Article Title: Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer.
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For WB: p300 (1:500, Invitrogen: MA1-16608, RW168, Vendor-validated); CBP (1:500, Invitrogen: PA5-27369, , Vendor-validated); AR (1:1000, Abcam: ab133273, ER179(2), Vendor-validated); H2BK5ac (1:1000, Cell Signaling Technology: 12799S, Vendor-validated); H2BK20ac (1:1000, Cell Signaling Technology: 34156S, vendor-validated); H2BK12ac (1:1000, Abcam: ab40883, EP858Y, Vendorvalidated); H2BK16ac (1:1000, Abcam: ab177427, EPR17598, Vendor-validated); H3K27ac (1:1000, Cell Signaling Technology: 8173S, D5E4, Vendor-validated); H3K18ac (1:1000, Active Motif: 39755, Vendor-validated); H3K4me1 (1:1000, Abcam: ab8895, Vendorvalidated); H3K4me3 (1:1000, Active Motif: 39060, Vendor-validated); H3K27me3(1:1000, Millipore: 07-449, Vendor-validated); H2B (1:1000, Active Motif: 39210, Vendor-validated); H3 (1:1000, Cell Signaling Technology: 3638S, 96C10, Vendor-validated); H2BK120ac (1:1000, Active Motif: 39119, Vendor-validated); Myc (1:1000, Cell Signaling Technology: 9402S, Vendor-validated); KLK3/PSA (1:1000, Dako: A0562, Vendor-validated); CITED2 (1:1000, Abcam: ab108345, EPR3416(2), Vendor-validated); NKX3-1 (1:1000, Cell Signaling Technology:83700S, D2Y1A, Vendor-validated); CCND1 (1:1000, Abcam: ab16663, SP4, Vendor-validated); FOXA1 (1:1000, Thermo Fisher Scientific: PA5-27157, Vendor-validated); Vinculin (1:1000, Cell Signaling Technology: 18799S, E1E9V, Vendorvalidated) ; GAPDH (1:1000, Santa Cruz Biotechnology: sc-47724, 0411, Vendor-validated); BRD2 (1:1000, Bethyl Laboratories: A700-008,BL-167-2A2, Vendor-validated); BRD3 (1:1000, Bethyl Laboratories: A302-368A, Vendor-validated); BRD4 (1:1000, Bethyl Laboratories: A700-004CF, BL-149-2H5, Vendor-validated); GSPT1 (1:1000, Proteintech: 28130-1-ap, Vendor-validated); Aiolos (1:1000, Cell Signaling Technology: 15103S, D1C1E, Vendor-validated); Ikaros (1:1000, Cell Signaling Technology: 14859S, D6N9Y, Vendor-validated), HDAC3 (1:1000, Abcam, ab137704, Vendor-validated), Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:10000, Biorad, #1706515), ECL Peroxidase (HRP) Anti-Mouse IgG (1:10000, Cytiva,NA931-100UL). .. For ChIP-seq: H3K27ac (1ug/million cells, Diagenode: C15410196, Vendor-validated); p300 (2ug/million cells, Abcam: ab14984, G230 / NM-11, Vendor-validated); H2BK20ac (1ug/million cells, Cell Signaling Technology: 34156S, vendor-validated); H2BK5ac (1ug/ million cells, Cell Signaling Technology: 12799S, Vendor-validated); H2BK16ac (1ug/million cells, Abcam, ab177427, EPR17598, Vendor-validated); AR (2ug/million cells, Millipore: 06-680, Vendor-validated); FOXA1 (2ug/million cells, Thermo Fisher Scientific: PA5-27157, Vendor-validated); BRD4 (2ug/million cells, Diagenode: C15410337, Vendor-validated); H3K18ac (1ug/million cells, Active Motif: 39755, Vendor-validated); MED1 (2ug/million cells, Active motif: 61065, Vendor-validated); ERG (2ug/million cells, Cell Signaling Technology: 97249S, A7L1G, Vendor-validated); RNA Pol II (1ug/million cells, Active motif: 39097, 4H8, Vendor-validated); Drosophila H2AV antibody for ChIP-seq Spike-in reference (1ug/25ug chromatin, Active Motif: 61686, Vendor-validated) For IHC or IF: p300 (1:250, Invitrogen: 33-7600, NM11, Vendor-validated); CBP (1:100, Invitrogen: PA5-27369, Vendor-validated); AR (1:4000, Abcam: ab133273, EPR1535(2)); Ki67 (predilute, Ventana Medical Systems: 790-4286, 30-9, Vendor-validated); H2BK20ac (1:50, Abcam: ab177430, epr859, Vendor-validated); H2BK5ac (1:250, Abcam: ab40886, Vendor-validated); CCND1 (predilute, Cell Marque: 241R-18, SP4, Vendor-validated); KRT8 (1:50, Abcam: ab53280, EP1628Y); H3K27ac (1:200, Cell Signaling Technology: 8173S, D5E4, Vendor-validated), Anti-rabbit HRP (predilute, .OmniMap, 760-4311), Anti-mouse HRP (predilute, OmniMap, 760-4310); nature portfolio | reporting sum m ary April 2023 4 nature portfolio | reporting sum m ary April 2023 Validation All antibodies used in this study are from reputed commercial vendors and have been validated by the vendors (see website). ..

    Immunohistochemistry:

    Article Title: Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For WB: p300 (1:500, Invitrogen: MA1-16608, RW168, Vendor-validated); CBP (1:500, Invitrogen: PA5-27369, , Vendor-validated); AR (1:1000, Abcam: ab133273, ER179(2), Vendor-validated); H2BK5ac (1:1000, Cell Signaling Technology: 12799S, Vendor-validated); H2BK20ac (1:1000, Cell Signaling Technology: 34156S, vendor-validated); H2BK12ac (1:1000, Abcam: ab40883, EP858Y, Vendorvalidated); H2BK16ac (1:1000, Abcam: ab177427, EPR17598, Vendor-validated); H3K27ac (1:1000, Cell Signaling Technology: 8173S, D5E4, Vendor-validated); H3K18ac (1:1000, Active Motif: 39755, Vendor-validated); H3K4me1 (1:1000, Abcam: ab8895, Vendorvalidated); H3K4me3 (1:1000, Active Motif: 39060, Vendor-validated); H3K27me3(1:1000, Millipore: 07-449, Vendor-validated); H2B (1:1000, Active Motif: 39210, Vendor-validated); H3 (1:1000, Cell Signaling Technology: 3638S, 96C10, Vendor-validated); H2BK120ac (1:1000, Active Motif: 39119, Vendor-validated); Myc (1:1000, Cell Signaling Technology: 9402S, Vendor-validated); KLK3/PSA (1:1000, Dako: A0562, Vendor-validated); CITED2 (1:1000, Abcam: ab108345, EPR3416(2), Vendor-validated); NKX3-1 (1:1000, Cell Signaling Technology:83700S, D2Y1A, Vendor-validated); CCND1 (1:1000, Abcam: ab16663, SP4, Vendor-validated); FOXA1 (1:1000, Thermo Fisher Scientific: PA5-27157, Vendor-validated); Vinculin (1:1000, Cell Signaling Technology: 18799S, E1E9V, Vendorvalidated) ; GAPDH (1:1000, Santa Cruz Biotechnology: sc-47724, 0411, Vendor-validated); BRD2 (1:1000, Bethyl Laboratories: A700-008,BL-167-2A2, Vendor-validated); BRD3 (1:1000, Bethyl Laboratories: A302-368A, Vendor-validated); BRD4 (1:1000, Bethyl Laboratories: A700-004CF, BL-149-2H5, Vendor-validated); GSPT1 (1:1000, Proteintech: 28130-1-ap, Vendor-validated); Aiolos (1:1000, Cell Signaling Technology: 15103S, D1C1E, Vendor-validated); Ikaros (1:1000, Cell Signaling Technology: 14859S, D6N9Y, Vendor-validated), HDAC3 (1:1000, Abcam, ab137704, Vendor-validated), Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:10000, Biorad, #1706515), ECL Peroxidase (HRP) Anti-Mouse IgG (1:10000, Cytiva,NA931-100UL). .. For ChIP-seq: H3K27ac (1ug/million cells, Diagenode: C15410196, Vendor-validated); p300 (2ug/million cells, Abcam: ab14984, G230 / NM-11, Vendor-validated); H2BK20ac (1ug/million cells, Cell Signaling Technology: 34156S, vendor-validated); H2BK5ac (1ug/ million cells, Cell Signaling Technology: 12799S, Vendor-validated); H2BK16ac (1ug/million cells, Abcam, ab177427, EPR17598, Vendor-validated); AR (2ug/million cells, Millipore: 06-680, Vendor-validated); FOXA1 (2ug/million cells, Thermo Fisher Scientific: PA5-27157, Vendor-validated); BRD4 (2ug/million cells, Diagenode: C15410337, Vendor-validated); H3K18ac (1ug/million cells, Active Motif: 39755, Vendor-validated); MED1 (2ug/million cells, Active motif: 61065, Vendor-validated); ERG (2ug/million cells, Cell Signaling Technology: 97249S, A7L1G, Vendor-validated); RNA Pol II (1ug/million cells, Active motif: 39097, 4H8, Vendor-validated); Drosophila H2AV antibody for ChIP-seq Spike-in reference (1ug/25ug chromatin, Active Motif: 61686, Vendor-validated) For IHC or IF: p300 (1:250, Invitrogen: 33-7600, NM11, Vendor-validated); CBP (1:100, Invitrogen: PA5-27369, Vendor-validated); AR (1:4000, Abcam: ab133273, EPR1535(2)); Ki67 (predilute, Ventana Medical Systems: 790-4286, 30-9, Vendor-validated); H2BK20ac (1:50, Abcam: ab177430, epr859, Vendor-validated); H2BK5ac (1:250, Abcam: ab40886, Vendor-validated); CCND1 (predilute, Cell Marque: 241R-18, SP4, Vendor-validated); KRT8 (1:50, Abcam: ab53280, EP1628Y); H3K27ac (1:200, Cell Signaling Technology: 8173S, D5E4, Vendor-validated), Anti-rabbit HRP (predilute, .OmniMap, 760-4311), Anti-mouse HRP (predilute, OmniMap, 760-4310); nature portfolio | reporting sum m ary April 2023 4 nature portfolio | reporting sum m ary April 2023 Validation All antibodies used in this study are from reputed commercial vendors and have been validated by the vendors (see website). ..

    Article Title: Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer.
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For WB: p300 (1:500, Invitrogen: MA1-16608, RW168, Vendor-validated); CBP (1:500, Invitrogen: PA5-27369, , Vendor-validated); AR (1:1000, Abcam: ab133273, ER179(2), Vendor-validated); H2BK5ac (1:1000, Cell Signaling Technology: 12799S, Vendor-validated); H2BK20ac (1:1000, Cell Signaling Technology: 34156S, vendor-validated); H2BK12ac (1:1000, Abcam: ab40883, EP858Y, Vendorvalidated); H2BK16ac (1:1000, Abcam: ab177427, EPR17598, Vendor-validated); H3K27ac (1:1000, Cell Signaling Technology: 8173S, D5E4, Vendor-validated); H3K18ac (1:1000, Active Motif: 39755, Vendor-validated); H3K4me1 (1:1000, Abcam: ab8895, Vendorvalidated); H3K4me3 (1:1000, Active Motif: 39060, Vendor-validated); H3K27me3(1:1000, Millipore: 07-449, Vendor-validated); H2B (1:1000, Active Motif: 39210, Vendor-validated); H3 (1:1000, Cell Signaling Technology: 3638S, 96C10, Vendor-validated); H2BK120ac (1:1000, Active Motif: 39119, Vendor-validated); Myc (1:1000, Cell Signaling Technology: 9402S, Vendor-validated); KLK3/PSA (1:1000, Dako: A0562, Vendor-validated); CITED2 (1:1000, Abcam: ab108345, EPR3416(2), Vendor-validated); NKX3-1 (1:1000, Cell Signaling Technology:83700S, D2Y1A, Vendor-validated); CCND1 (1:1000, Abcam: ab16663, SP4, Vendor-validated); FOXA1 (1:1000, Thermo Fisher Scientific: PA5-27157, Vendor-validated); Vinculin (1:1000, Cell Signaling Technology: 18799S, E1E9V, Vendorvalidated) ; GAPDH (1:1000, Santa Cruz Biotechnology: sc-47724, 0411, Vendor-validated); BRD2 (1:1000, Bethyl Laboratories: A700-008,BL-167-2A2, Vendor-validated); BRD3 (1:1000, Bethyl Laboratories: A302-368A, Vendor-validated); BRD4 (1:1000, Bethyl Laboratories: A700-004CF, BL-149-2H5, Vendor-validated); GSPT1 (1:1000, Proteintech: 28130-1-ap, Vendor-validated); Aiolos (1:1000, Cell Signaling Technology: 15103S, D1C1E, Vendor-validated); Ikaros (1:1000, Cell Signaling Technology: 14859S, D6N9Y, Vendor-validated), HDAC3 (1:1000, Abcam, ab137704, Vendor-validated), Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:10000, Biorad, #1706515), ECL Peroxidase (HRP) Anti-Mouse IgG (1:10000, Cytiva,NA931-100UL). .. For ChIP-seq: H3K27ac (1ug/million cells, Diagenode: C15410196, Vendor-validated); p300 (2ug/million cells, Abcam: ab14984, G230 / NM-11, Vendor-validated); H2BK20ac (1ug/million cells, Cell Signaling Technology: 34156S, vendor-validated); H2BK5ac (1ug/ million cells, Cell Signaling Technology: 12799S, Vendor-validated); H2BK16ac (1ug/million cells, Abcam, ab177427, EPR17598, Vendor-validated); AR (2ug/million cells, Millipore: 06-680, Vendor-validated); FOXA1 (2ug/million cells, Thermo Fisher Scientific: PA5-27157, Vendor-validated); BRD4 (2ug/million cells, Diagenode: C15410337, Vendor-validated); H3K18ac (1ug/million cells, Active Motif: 39755, Vendor-validated); MED1 (2ug/million cells, Active motif: 61065, Vendor-validated); ERG (2ug/million cells, Cell Signaling Technology: 97249S, A7L1G, Vendor-validated); RNA Pol II (1ug/million cells, Active motif: 39097, 4H8, Vendor-validated); Drosophila H2AV antibody for ChIP-seq Spike-in reference (1ug/25ug chromatin, Active Motif: 61686, Vendor-validated) For IHC or IF: p300 (1:250, Invitrogen: 33-7600, NM11, Vendor-validated); CBP (1:100, Invitrogen: PA5-27369, Vendor-validated); AR (1:4000, Abcam: ab133273, EPR1535(2)); Ki67 (predilute, Ventana Medical Systems: 790-4286, 30-9, Vendor-validated); H2BK20ac (1:50, Abcam: ab177430, epr859, Vendor-validated); H2BK5ac (1:250, Abcam: ab40886, Vendor-validated); CCND1 (predilute, Cell Marque: 241R-18, SP4, Vendor-validated); KRT8 (1:50, Abcam: ab53280, EP1628Y); H3K27ac (1:200, Cell Signaling Technology: 8173S, D5E4, Vendor-validated), Anti-rabbit HRP (predilute, .OmniMap, 760-4311), Anti-mouse HRP (predilute, OmniMap, 760-4310); nature portfolio | reporting sum m ary April 2023 4 nature portfolio | reporting sum m ary April 2023 Validation All antibodies used in this study are from reputed commercial vendors and have been validated by the vendors (see website). ..

    Biomarker Discovery:

    Article Title: Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For WB: p300 (1:500, Invitrogen: MA1-16608, RW168, Vendor-validated); CBP (1:500, Invitrogen: PA5-27369, , Vendor-validated); AR (1:1000, Abcam: ab133273, ER179(2), Vendor-validated); H2BK5ac (1:1000, Cell Signaling Technology: 12799S, Vendor-validated); H2BK20ac (1:1000, Cell Signaling Technology: 34156S, vendor-validated); H2BK12ac (1:1000, Abcam: ab40883, EP858Y, Vendorvalidated); H2BK16ac (1:1000, Abcam: ab177427, EPR17598, Vendor-validated); H3K27ac (1:1000, Cell Signaling Technology: 8173S, D5E4, Vendor-validated); H3K18ac (1:1000, Active Motif: 39755, Vendor-validated); H3K4me1 (1:1000, Abcam: ab8895, Vendorvalidated); H3K4me3 (1:1000, Active Motif: 39060, Vendor-validated); H3K27me3(1:1000, Millipore: 07-449, Vendor-validated); H2B (1:1000, Active Motif: 39210, Vendor-validated); H3 (1:1000, Cell Signaling Technology: 3638S, 96C10, Vendor-validated); H2BK120ac (1:1000, Active Motif: 39119, Vendor-validated); Myc (1:1000, Cell Signaling Technology: 9402S, Vendor-validated); KLK3/PSA (1:1000, Dako: A0562, Vendor-validated); CITED2 (1:1000, Abcam: ab108345, EPR3416(2), Vendor-validated); NKX3-1 (1:1000, Cell Signaling Technology:83700S, D2Y1A, Vendor-validated); CCND1 (1:1000, Abcam: ab16663, SP4, Vendor-validated); FOXA1 (1:1000, Thermo Fisher Scientific: PA5-27157, Vendor-validated); Vinculin (1:1000, Cell Signaling Technology: 18799S, E1E9V, Vendorvalidated) ; GAPDH (1:1000, Santa Cruz Biotechnology: sc-47724, 0411, Vendor-validated); BRD2 (1:1000, Bethyl Laboratories: A700-008,BL-167-2A2, Vendor-validated); BRD3 (1:1000, Bethyl Laboratories: A302-368A, Vendor-validated); BRD4 (1:1000, Bethyl Laboratories: A700-004CF, BL-149-2H5, Vendor-validated); GSPT1 (1:1000, Proteintech: 28130-1-ap, Vendor-validated); Aiolos (1:1000, Cell Signaling Technology: 15103S, D1C1E, Vendor-validated); Ikaros (1:1000, Cell Signaling Technology: 14859S, D6N9Y, Vendor-validated), HDAC3 (1:1000, Abcam, ab137704, Vendor-validated), Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:10000, Biorad, #1706515), ECL Peroxidase (HRP) Anti-Mouse IgG (1:10000, Cytiva,NA931-100UL). .. For ChIP-seq: H3K27ac (1ug/million cells, Diagenode: C15410196, Vendor-validated); p300 (2ug/million cells, Abcam: ab14984, G230 / NM-11, Vendor-validated); H2BK20ac (1ug/million cells, Cell Signaling Technology: 34156S, vendor-validated); H2BK5ac (1ug/ million cells, Cell Signaling Technology: 12799S, Vendor-validated); H2BK16ac (1ug/million cells, Abcam, ab177427, EPR17598, Vendor-validated); AR (2ug/million cells, Millipore: 06-680, Vendor-validated); FOXA1 (2ug/million cells, Thermo Fisher Scientific: PA5-27157, Vendor-validated); BRD4 (2ug/million cells, Diagenode: C15410337, Vendor-validated); H3K18ac (1ug/million cells, Active Motif: 39755, Vendor-validated); MED1 (2ug/million cells, Active motif: 61065, Vendor-validated); ERG (2ug/million cells, Cell Signaling Technology: 97249S, A7L1G, Vendor-validated); RNA Pol II (1ug/million cells, Active motif: 39097, 4H8, Vendor-validated); Drosophila H2AV antibody for ChIP-seq Spike-in reference (1ug/25ug chromatin, Active Motif: 61686, Vendor-validated) For IHC or IF: p300 (1:250, Invitrogen: 33-7600, NM11, Vendor-validated); CBP (1:100, Invitrogen: PA5-27369, Vendor-validated); AR (1:4000, Abcam: ab133273, EPR1535(2)); Ki67 (predilute, Ventana Medical Systems: 790-4286, 30-9, Vendor-validated); H2BK20ac (1:50, Abcam: ab177430, epr859, Vendor-validated); H2BK5ac (1:250, Abcam: ab40886, Vendor-validated); CCND1 (predilute, Cell Marque: 241R-18, SP4, Vendor-validated); KRT8 (1:50, Abcam: ab53280, EP1628Y); H3K27ac (1:200, Cell Signaling Technology: 8173S, D5E4, Vendor-validated), Anti-rabbit HRP (predilute, .OmniMap, 760-4311), Anti-mouse HRP (predilute, OmniMap, 760-4310); nature portfolio | reporting sum m ary April 2023 4 nature portfolio | reporting sum m ary April 2023 Validation All antibodies used in this study are from reputed commercial vendors and have been validated by the vendors (see website). ..

    Article Title: Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer.
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For WB: p300 (1:500, Invitrogen: MA1-16608, RW168, Vendor-validated); CBP (1:500, Invitrogen: PA5-27369, , Vendor-validated); AR (1:1000, Abcam: ab133273, ER179(2), Vendor-validated); H2BK5ac (1:1000, Cell Signaling Technology: 12799S, Vendor-validated); H2BK20ac (1:1000, Cell Signaling Technology: 34156S, vendor-validated); H2BK12ac (1:1000, Abcam: ab40883, EP858Y, Vendorvalidated); H2BK16ac (1:1000, Abcam: ab177427, EPR17598, Vendor-validated); H3K27ac (1:1000, Cell Signaling Technology: 8173S, D5E4, Vendor-validated); H3K18ac (1:1000, Active Motif: 39755, Vendor-validated); H3K4me1 (1:1000, Abcam: ab8895, Vendorvalidated); H3K4me3 (1:1000, Active Motif: 39060, Vendor-validated); H3K27me3(1:1000, Millipore: 07-449, Vendor-validated); H2B (1:1000, Active Motif: 39210, Vendor-validated); H3 (1:1000, Cell Signaling Technology: 3638S, 96C10, Vendor-validated); H2BK120ac (1:1000, Active Motif: 39119, Vendor-validated); Myc (1:1000, Cell Signaling Technology: 9402S, Vendor-validated); KLK3/PSA (1:1000, Dako: A0562, Vendor-validated); CITED2 (1:1000, Abcam: ab108345, EPR3416(2), Vendor-validated); NKX3-1 (1:1000, Cell Signaling Technology:83700S, D2Y1A, Vendor-validated); CCND1 (1:1000, Abcam: ab16663, SP4, Vendor-validated); FOXA1 (1:1000, Thermo Fisher Scientific: PA5-27157, Vendor-validated); Vinculin (1:1000, Cell Signaling Technology: 18799S, E1E9V, Vendorvalidated) ; GAPDH (1:1000, Santa Cruz Biotechnology: sc-47724, 0411, Vendor-validated); BRD2 (1:1000, Bethyl Laboratories: A700-008,BL-167-2A2, Vendor-validated); BRD3 (1:1000, Bethyl Laboratories: A302-368A, Vendor-validated); BRD4 (1:1000, Bethyl Laboratories: A700-004CF, BL-149-2H5, Vendor-validated); GSPT1 (1:1000, Proteintech: 28130-1-ap, Vendor-validated); Aiolos (1:1000, Cell Signaling Technology: 15103S, D1C1E, Vendor-validated); Ikaros (1:1000, Cell Signaling Technology: 14859S, D6N9Y, Vendor-validated), HDAC3 (1:1000, Abcam, ab137704, Vendor-validated), Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:10000, Biorad, #1706515), ECL Peroxidase (HRP) Anti-Mouse IgG (1:10000, Cytiva,NA931-100UL). .. For ChIP-seq: H3K27ac (1ug/million cells, Diagenode: C15410196, Vendor-validated); p300 (2ug/million cells, Abcam: ab14984, G230 / NM-11, Vendor-validated); H2BK20ac (1ug/million cells, Cell Signaling Technology: 34156S, vendor-validated); H2BK5ac (1ug/ million cells, Cell Signaling Technology: 12799S, Vendor-validated); H2BK16ac (1ug/million cells, Abcam, ab177427, EPR17598, Vendor-validated); AR (2ug/million cells, Millipore: 06-680, Vendor-validated); FOXA1 (2ug/million cells, Thermo Fisher Scientific: PA5-27157, Vendor-validated); BRD4 (2ug/million cells, Diagenode: C15410337, Vendor-validated); H3K18ac (1ug/million cells, Active Motif: 39755, Vendor-validated); MED1 (2ug/million cells, Active motif: 61065, Vendor-validated); ERG (2ug/million cells, Cell Signaling Technology: 97249S, A7L1G, Vendor-validated); RNA Pol II (1ug/million cells, Active motif: 39097, 4H8, Vendor-validated); Drosophila H2AV antibody for ChIP-seq Spike-in reference (1ug/25ug chromatin, Active Motif: 61686, Vendor-validated) For IHC or IF: p300 (1:250, Invitrogen: 33-7600, NM11, Vendor-validated); CBP (1:100, Invitrogen: PA5-27369, Vendor-validated); AR (1:4000, Abcam: ab133273, EPR1535(2)); Ki67 (predilute, Ventana Medical Systems: 790-4286, 30-9, Vendor-validated); H2BK20ac (1:50, Abcam: ab177430, epr859, Vendor-validated); H2BK5ac (1:250, Abcam: ab40886, Vendor-validated); CCND1 (predilute, Cell Marque: 241R-18, SP4, Vendor-validated); KRT8 (1:50, Abcam: ab53280, EP1628Y); H3K27ac (1:200, Cell Signaling Technology: 8173S, D5E4, Vendor-validated), Anti-rabbit HRP (predilute, .OmniMap, 760-4311), Anti-mouse HRP (predilute, OmniMap, 760-4310); nature portfolio | reporting sum m ary April 2023 4 nature portfolio | reporting sum m ary April 2023 Validation All antibodies used in this study are from reputed commercial vendors and have been validated by the vendors (see website). ..



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